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嗜热真菌Neosartorya fischeri P1脂肪酶基因的克隆、表达及酶学性质分析
从云南酸矿废水中获得了一株脂肪酶活性较高的嗜热真菌,经显微形态及转录间隔区(ITS)序列分析鉴定为新萨托菌,命名为Neosartorya fischeri P1。通过同源克隆,从该真菌中获得了脂肪酶基因Lip024,并在Escherichia coli BL21(DE3)和Pichia pastoris GS115中进行表达,表达量分别为0.09 U/mL、0.26Expand
Comparison of the proteome of Mycobacterium tuberculosis cytosolic basic proteins betwen multiple drugresistant stainn and sensitive stains
目的 研究结核分枝杆菌耐多药菌株与敏感菌株菌体碱性蛋白的蛋白质表达差异.方法 运用双向电泳技术(2-DE)比较8株耐多药菌株与9株敏感菌株的菌体碱性蛋白表达图谱差异,差异蛋白进行胶内酶切,肽混合物使用基质辅助激光解吸-电离飞行时间质谱仪(MALDI-TOF-MS)进行质谱分析,将肽质量指纹谱数据输入互联网上的蛋白质数据库进行检索.结果Expand
Red date and black tea fungus beverage and its brewing method
Disclosed is a femented beverage with black tea bacterium as the bacterial and its brewing process, which employs by-product of red date or jujube fabrication as the raw material, and utilizesExpand
Strain produced by beta-glucosidase and method for preparing Genipin therefrom
The invention relates to a method for effectively converting Geniposide into Genipin by stimulating the conversion of microbes in the in vivo intestinal tract through an in vitro microbialExpand
碱性木聚糖酶产生菌的筛选、XynG1-3基因克隆表达及酶学性质研究
从造纸厂周边碱性土壤中分离、筛选到一株产碱性木聚糖酶的细菌G1-3,根据形态和生理、生化特性并结合16SrDNA序列分析,鉴定菌株G1-3为短小芽孢杆菌,命名为Bacillus pumilusG1-3。利用克隆表达载体pET-22b(+),实现了BacillusExpand
Preparation method for phosphatidylserine synthetase
The invention belongs to the bioengineering field, and relates to a preparation method for a phosphatidylserine synthetase. The technical proposal comprises the following steps of: fermenting andExpand
纳豆咀嚼片中微量元素铁、锌、铜、锰的分析
采用湿法消化样品,确定了检测纳豆咀嚼片微量元素的最佳处理条件。试验证明,混酸HNO3:HClO4的比例为4:1,消化温度为140℃,消化时间80min时样品可以得到充分分解。采用了ICP-AES法测定纳豆咀嚼片中微量元素铁、锌、铜、锰的含量,测定结果比较准确。
Rhizopus chinensis 12^#发酵产生纤溶酶的分离提纯
分离自南方小酒药的华根霉12#(Rhizopus chinensis 12#)以豆粕和麸皮为原料固体发酵可产生多种纤溶活性组分.采用饱和度40%~70%的硫酸铵溶液分步盐析、Octyl Sepharose Fast Flow疏水层析、Q-Sepharose High Performance 离子交换层析和Sephacryl S-100Expand