• Publications
  • Influence
嗜热真菌Neosartorya fischeri P1脂肪酶基因的克隆、表达及酶学性质分析
从云南酸矿废水中获得了一株脂肪酶活性较高的嗜热真菌,经显微形态及转录间隔区(ITS)序列分析鉴定为新萨托菌,命名为Neosartorya fischeri P1。通过同源克隆,从该真菌中获得了脂肪酶基因Lip024,并在Escherichia coli BL21(DE3)和Pichia pastoris GS115中进行表达,表达量分别为0.09 U/mL、0.26Expand
α1,2-岩藻糖基转移酶基因的克隆及在大肠杆菌中的表达
利用PCR方法从幽门螺旋杆菌(Helicobacter pylori,HP)基因组DNA中获得α1,2-岩藻糖基转移酶(α1,2-fuco-syltransferase,α1,2-fuct)基因,得到大小为906 bp的目的基因,将其定向插入到原核表达载体pET-22b(+)中,得到重组表达载体pET-fuct。将重组表达载体转化到大肠杆菌BL21(DE3)中,25℃,0.1Expand
Comparison of the proteome of Mycobacterium tuberculosis cytosolic basic proteins betwen multiple drugresistant stainn and sensitive stains
TLDR
Familiarity, ease of access, trust, and awareness of benefits and risks are important. Expand
Red date and black tea fungus beverage and its brewing method
Disclosed is a femented beverage with black tea bacterium as the bacterial and its brewing process, which employs by-product of red date or jujube fabrication as the raw material, and utilizesExpand
碱性木聚糖酶产生菌的筛选、XynG1-3基因克隆表达及酶学性质研究
从造纸厂周边碱性土壤中分离、筛选到一株产碱性木聚糖酶的细菌G1-3,根据形态和生理、生化特性并结合16SrDNA序列分析,鉴定菌株G1-3为短小芽孢杆菌,命名为Bacillus pumilusG1-3。利用克隆表达载体pET-22b(+),实现了BacillusExpand
Strain produced by beta-glucosidase and method for preparing Genipin therefrom
The invention relates to a method for effectively converting Geniposide into Genipin by stimulating the conversion of microbes in the in vivo intestinal tract through an in vitro microbialExpand
纳豆咀嚼片中微量元素铁、锌、铜、锰的分析
采用湿法消化样品,确定了检测纳豆咀嚼片微量元素的最佳处理条件。试验证明,混酸HNO3:HClO4的比例为4:1,消化温度为140℃,消化时间80min时样品可以得到充分分解。采用了ICP-AES法测定纳豆咀嚼片中微量元素铁、锌、铜、锰的含量,测定结果比较准确。
Preparation method for phosphatidylserine synthetase
The invention belongs to the bioengineering field, and relates to a preparation method for a phosphatidylserine synthetase. The technical proposal comprises the following steps of: fermenting andExpand
...
1
2
3
4
...