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  • 蔺芳, 付瑞敏, +4 authors 陈慧珍
  • 2011
  • 基于SYBR Green I荧光染料与双链DNA结合产生荧光的原理,建立一种痕量DNA荧光检测方法。采用不同稀释倍数的SYBR Green I荧光染料做DNA的标准曲线,确定SYBR Green I荧光染料的最佳稀释倍数。比较荧光法和Southern blotting杂交法定量DNA。结果表明,SYBR Green I荧光染料稀释倍数为1∶10Continue Reading