• Publications
  • Influence
耐旱植物厚叶旋蒴苣苔 BDN1 脱水素基因的克隆及表达特性分析
根据已知植物脱水素基因的保守区域设计引物,采用RT-PCR方法从耐旱植物厚叶旋蒴苣苔的总cDNA中扩增出一个500 bp的与植物脱水素基因同源的序列.结合5′RACE获得了植物脱水素基因家族一个新成员 BDN1 的全长cDNA(1148bp). Southern杂交分析表明, BDN1 基因在厚叶旋蒴苣苔基因组中以单拷贝形式存在.Expand
  • 1
Cladosporium endogenic fungus capable of producing veralkol
Fungi cloned stilbene synthase gene (sts) within the present invention from green ivy (Parthenocissi Tricuspidatae), it ivy sts gene coding sequence similarity of 95.25% by 18SrDNA sequenceExpand
Leafy启动子控制下5-烯醇式丙酮酰-莽草酸-3-磷酸合酶基因(CP4EPSPS)的表达增强芽对草甘膦的抗性
抗草甘膦基因在转基因植物体内持续高效表达,不但增加植物代谢压力,有的甚至改变植物形态造成植物的生长发育畸形。为了减少转基因植株的代谢负担和能源浪费,从拟南芥菜(ArabidopsisExpand
Anhydrant gene BcDh1 and the application of its promoter in raising drought-enduring plant
The present invention clones one new anhydrant gene of 759 bp length ov Baoea crassifolia Hemsl. and its 5' end controlling sequence 1159 bp. The gene is the SK2 type member in the anhydrant familyExpand
锚定PCR(Anchored PCR): 一种新的染色体步行方法
基于PCR的技术是克隆已知DNA片段侧翼序列的最常用方法.到目前为止,这些方法大致可以分为3种类型:反向PCR(inverse PCR)、连接介导的PCR(1igation-mediated PCR)和随机引物PCR(randomly primed PCR).反向PCR是使用最早的方法,其原理是用限制性内切酶消化基因组总
转基因马铃薯中病原诱导 GO 基因的表达及其对晚疫病的抗性
利用PCR技术从黑曲霉( Aspergillus niger )中扩增并克隆了葡萄糖氧化酶(glucose oxidase, 简称GO)基因,并将马铃薯病原诱导型启动子( Prp1-1 基因启动子)与其融合构建了植物表达载体pCAMGO.经农杆菌介导转化获得了转基因植株,Southern杂交显示葡萄糖氧化酶基因已经整合进马铃薯基因组.该基因的表达及所引起的H 2 O 2Expand
Use of orychophragmus violaceus ODREB2B gene for cultivating drought-tolerant plant
The invention clones a 978bp drought-related duplicating factor coding gene (ODREB2B) sequence of Orychohragmus violaceus, also called Feb orchid. The gene coded product is a new drought-inducedExpand