Amycolatopsis orientalis由来エキソ-β-グルコサミニダーゼの構造と機能
- 深溝 慶, R. Brzezinski
- Biology, Chemistry
- 20 April 2007
To identify the catalytic residue, mutations were introduced into the putative catalytic residues resulting in five mutated enzymes which were successfully produced, indicating that Asp469 and Glu541 are essential for catalysis as predicted from sequence alignment of enzymes belonging to GH-2 family.
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