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在碱性介质中,三与硫胺素反应产生荧光物质,表面活性剂TritonX-100地该反应具有敏化作用。据此建立了测定微量然的荧光分析法,其荧光光谱的激光波长λcx=375nm发射波长λnm=440nm。在6.0×10^-8-2.4×10^-7mol/L范围内荧光强度与钴离子的浓度呈良好线性关系。将其用于合金钢和维生素B12针剂中微量钴的测定,取得了满意结果。并对发光机理进行了探讨。