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  • 韩正祥, 张梦瑾, +4 authors 徐开林
  • 2015
  • 目的:构建带有GST标签的人NRP-1融合蛋白原核表达载体,在大肠埃希菌( E.coli)中诱导其表达,并进行包涵体的复性及纯化。方法:利用RT-PCR扩增人NRP-1基因,将其克隆至pCR-blunt载体,酶切制备NRP-1基因片段,插入表达载体pGEX-4T-1,生成pGEX-4T-1-NRP-1,转入BL21感受态细胞以IPTG诱导其蛋白的表达。裂解细菌后行WesternContinue Reading
  • 张梦瑾, Zhang Mengjin, +7 authors Han Zheng-xiang
  • 2016 (First Published: 25 August 2016)
  • 目的 通过上调乳腺癌细胞MDA-MB-231、SK-BR-3中NRP-1的表达,观察NRP-1对细胞增殖、凋亡、迁移及侵袭能力的影响.方法 构建pcDNA3.1-NRP-1表达载体,脂质体介导NRP-1表达质粒转染MDA-MB-231、SK-BR-3细胞,用G418筛选出稳定转染的乳腺癌细胞株.利用RT-qPCR、WesternContinue Reading