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目的:观察转化生长因子β1( TGF-β1)对重组骨形态蛋白2( rhBMP-2)促进大鼠骨髓间充质干细胞增殖分化的影响。方法分离培养骨髓间充质干细胞,然后加入不同浓度的rhBMP-2,用MTT法检测细胞增殖情况,通过ELISA法检测细胞碱性磷酸酶( ALP)活性评估其骨向分化能力。同时加入TGF-β1观察其对rh-BMP-2协同促进能力。结果不同浓度rhBMP-2对骨髓间充质干细胞(Continue Reading