Štefan Bauer

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Four mutant strains exhibiting increased production of cullulases were prepared by UV irradiation of conidia ofTrichoderma viride QM 9414. Selected mutants were tested for production of cellulases in submerged cultivations in shake flasks and in a 30-L fermentor in a synthetic medium containing 1 % microcrystaline cellulose as the carbon source. Some(More)
Water-soluble extracellular polysaccharide-protein complexes of a molecular weight of about 200,000 were isolated from glucose-containing nutrient media after cultivation of slightly, moderately and highly virulent strains ofCandida albicans (Robin) Berkhout. Chemical, physico-chemical and immunological properties of these compounds were studied. The(More)
The cellulolytic enzyme complexes secreted by the fungus Trichoderma reesei QM 9414 and its mutants M 5, M 6, MHC 15, and MHC 22 were characterized by determining their specific filter-paper (FP)-, carboxymethylcellulase (Cx)-and β-glucosidase (βG)-activities. They were characterised further by measuring their Cx and βG profiles after separation on an(More)
Selection of a large number of different strains of hyphal fungi of the genusAspergillus, capable of production of extracellular mannosidase and mannanase type enzymes, was carried out. Before cultivating the strains on liquid synthetic medium containing 0.5%Saccharomyces cerevisiae mannan as the carbon source, they were adapted by multiple passage on solid(More)
The paper describes isolation and partial characterization of the tissue culture of the common spruce (Picea excelsa Link). The tissue culture was grown on the medium ofHarvey (1967) modified by increasing the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) to l mg l−1 and by omitting the tyrosine. By selective 10-month subculturing the tissue(More)
The combination of high-resolution autoradiography and biochemical methods has been used to ascertain the site of mannan synthesis in the yeastSaccharomyces cerevisiae. High-resolution autoradiography has been performed under conditions when addedd-mannose-3H was incorporated exclusively into mannan. Application of “pulse-chase” labelling technique revealed(More)
StrainAureobasidium pullulans capable of utilizing hemicelluloses and xylan was cultivated on processed waste dialysis liquor from the production of viscose fibres, containing about 1.5 % hemicelluloses. Basic conditions of biomass production were tested on a laboratory scale. The dialysis waste liquor adjusted with mineral acids to pH 4 — 5 and(More)