Attempts to immunize sheep against Haemonchus contortus using a cocktail of recombinant proteases derived from the protective antigen, H‐gal‐GP
- É. Cachat, G. Newlands, S. E. Ekoja, H. McAllister, W. Smith
- BiologyParasite immunology (Print)
- 1 June 2010
High levels of serum antibodies that recognized H‐gal‐GP were detected in both the native antigen and recombinantly expressed cocktail‐immunized groups by the time of challenge, but protective immunity was only observed in the group immunized with native H‐Gal‐GP.
A Bacillus thuringiensis strain producing a polyglutamate capsule resembling that of Bacillus anthracis.
Bacillus thuringiensis serovar Monterrey strain BGSC 4AJ1 produced a microscopically visible capsule that reacted with a fluorescent antibody specific for the poly-gamma-d-glutamic acid (PGA) capsule…
Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells
- J. Baaske, P. Gonschorek, M. Zurbriggen
- BiologyScientific Reports
- 9 October 2018
This work presents a novel two-component, blue light-responsive optogenetic OFF switch (‘Blue-OFF’), which enables a rapid and quantitative down-regulation of a protein upon illumination, and outperforms current optogenetics systems for controlling protein levels.
Synthetic biology meets tissue engineering
The ‘embryological cycle’ of patterning, differentiation and morphogenesis is outlined and progress that has been made in constructing synthetic biological systems to reproduce these processes in new ways are reviewed.
Lactobacillus suntoryeus sp. nov., isolated from malt whisky distilleries.
Eight strains of Lactobacillus with identical partial 16S rRNA gene sequences and similar randomly amplified polymorphic DNA patterns were isolated from fermentation samples from Japanese and Scottish malt whisky distilleries, suggesting different relationships among the strains and the distillery isolates no longer formed a monophyletic group.
2- and 3-dimensional synthetic large-scale de novo patterning by mammalian cells through phase separation
- É. Cachat, Weijia Liu, J. Davies
- BiologyScientific Reports
- 9 February 2016
This work describes a synthetic biological system that confers large-scale de novo patterning activity on 2-D and 3-D populations of mammalian cells, inspired by the known phenomenon of cadherin-based sorting.
A library of mammalian effector modules for synthetic morphology
- É. Cachat, Weijia Liu, P. Hohenstein, J. Davies
- Biology, EngineeringJournal of Biological Engineering
- 19 November 2014
A modular library of synthetic morphogenetic driver genes to control (separately) mammalian cell adhesion, locomotion, fusion, proliferation and elective cell death is assembled and its use in the T-REx-293 human cell line is demonstrated.
The art of fusion: A synthetic approach to create cross-kingdom hybrids
- É. Cachat, Leonard J Nelson, Oron Catts
- Biology
- 2018
Synthetic self-patterning and morphogenesis in mammalian cells: a proof-of-concept step towards synthetic tissue development
- É. Cachat, Weijia Liu, J. Davies
- Biology
- 1 September 2017
The authors have constructed and published a synthetic module that drives self-organised patterning of mammalian cell populations into patches of different cell types and extend the self-patterning module with a morphogenetic effector that drives elective cell death in just one cell type.
Application of synthetic biology to regenerative medicine
It is argued that bringing these young fields of synthetic biology and regenerative medicine together, so that synthetic biology techniques are applied to the problem of regeneration, has the potential significantly to enhance the authors' ability to help those in clinical need.
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